• General Overview of AdiStem Technology - English_Vasilis Paspaliaris
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PROCEDURE OVERVIEW
The following is a brief overview of Adult Stem Cell Therapy using Adipose or Fat derived stem cells. The fat stem cells come from the patients own abdominal region. There are about 1-2 million stem cells and regenerative cells in 1 cc of pure fat.

 History 

For over 60 years, Stem Cells have been used to treat patients with conditions such as Leukemia and Lymph Node Cancer. This is know as a bone marrow transplant, using Mesenchymal Stem Cells ( MSC’s) . Today, these MSC’s have been found and are now harvested from the patients own fat, activated and simply returned. These Fat derived stem cells are used in the treatment of a wide range of conditions with positive and encouraging results.

brief-overview

  Adipose tissue contains significantly more stem cells

            per cc (ml) of  tissue than bone marrow

Steps of Fat Stem Cell Therapy

  1. A small quantity of fat is taken from a person using a mino-lipo procedure, about 150cc.
  2. These harvested cells (usually 100-250 million), are isolated from the fat using a dissolving solution. The cells are then concentrated and activated in a standard centrifuge. Next they are put in a normal saline drip and given back to the patient. This is called autologous (patient’s own stem cells and DNA) cell therapy.
    SVF   The Stromal Vascular Fraction (SVF) – Stem Cells ( larger ) plus other regenerative cells make up this group of “repair cell”.
  3. The stem cells go to work naturally repairing the damaged tissue or organ over time, usually 6 to 9 months.
  4. If stem cell banking or cryopreservation is available, extra fat can be taken, the cell isolated, divided into doses and given at a later date when needed.

PHOTOMODULATION    

Perhaps the next generation of urilizing our own body’s to self heal may utilize a low level laser applied to PRP or platelet rich plasma or bone marrow aspirate (BMAC). After the PRP is prepared and is an a syringe, it is then placed in a device that helps communicate the the cells & steer them towards an anti-inflammatory pathway.
The company AdiStem Ltd., which operates laboratories worldwide, has been researching how monochromatic light of various frequencies and intensities affects populations of mesenchyme stem cells and white blood cells in animals as well as in humans.

Photomodulation and photoactivation using low-level light is used to stimulate and control growth factors (the key component of PRP) in living cells. These lights block the pro-inflammation activity of proteins called cytokines. These Cytokines have been identified as key players in osteoarthritis and chronic tendon injuries and wounds.

Selecting the optimal photomodulaiton wavelength and bandwidth are critical in creating the ideal results. This light stimulation of cells results in the production or inhibition of ATP, which provides the cell with energy and proliferation.

AdiLight-1 is the first commercially available LED device for mesenchyme stem cell activation and the modulation of cytokine release.

Mesenchyme Stem Cells

When mesenchyme stem cells derived from adipose (fat) tissue are removed from a subject most of the cells will lie dormant. Stem cells and progenitor cells in the body are activated by a cascade of physiological processes such as the release of chemokines and growth factor from platelets in the blood. When photons from the AdiLight-1 act on stem cells derived from adipose for 20 minutes we observe an increase in integrin production, interleukin 1 recptor antagonist, thymosin beta 4, vascular endothelial growth factor, and of course, proliferation (see Figure 1). Most importantly the clinical results of the AdiLight-1 are consistent across age groups.

photomodulation-2

 

Figure 1. Colonies of stem cells derived from adipose – untreated (left). Colonies of stem cells derived from adipose after 20-minute

AdiLight-1 exposure before plating (right). 

Peripheral Blood White Blood Cells

Internal medicine specialists throughout Korea and Europe have been utilizing this therapy via photoactivation for many years. White blood cells, exposed to AdiLight-1 for 10-minutes show an anti-inflammatory cytokine response. (See Figure 2). We postulate that using the Adistem light therapy on prepared PRP may potentially result in less post injection pain and quicker recovery by guiding the cells to an anti vs pro-inflammation pathway. We find that some patients have more pain post treatment vs. others & this adjunct allows us to steer patients towards a more speedy recovery. More research is underway to better understand how laser light therapy may further assist our cells to heal.

photomodulation-3

 

Figure 2. Results in four patients after a 10-minute exposure to AdiLight-1. Elisa assay for

plasma interleukin 1 receptor antagonist peripheral WBCs before (left) and after (right). 

Internal medicine specialists throughout Korea and Europe have been utilizing this therapy via photoactivation for many years. White blood cells, exposed to AdiLight-1 for 10-minutes show an anti-inflammatory cytokine response. (See Figure 2). We postulate that using the Adistem light therapy on prepared PRP may potentially result in less post injection pain and quicker recovery by guiding the cells to an anti vs pro-inflammation pathway. We find that some patients have more pain post treatment vs. others & this adjunct allows us to steer patients towards a more speedy recovery. More research is underway to better understand how laser light therapy may further assist our cells to heal.

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