Osteoarthritis (OA) is a progressive degenerative condition that affect the quality of life for many patients. The disease results in pain and stiffness that leads to significant physical and psychological disability, and adversely affects daily function and quality of life, often leading to surgery including hip and knee replacements. Now, biotechnology and adult stem cell therapy can offer safe and clinically proven cell therapies to patient with joint pain. These treatment use your own cell from your blood or adipose tissue to help heal your hip, knee, shoulder, back or neck. The latest EU and USA stem cell technologies are available in Bangkok, Thailand.
Recent research has demonstrated that Human adipose-derived stem cells (ADSCs) can differentiate into cartilage and bone suggesting a potential treatment in osteoarthritis (Diekman et al, 2010; Kern et al 2006). Indeed, cartilage regrowth has been shown in animal models with cartilage defects (Dragoo et al, 2007; Cui et al, 2009) and research in Humans has demonstrated its safety and efficacy in phase I and II clinical trials with up to two years follow up (Garcia-Olmo et al, 2005; Garcia-Olmo et al, 2008; Garcia-Olmo et al, 2009). Further, the sampling of cells from adipose tissue rather than bone marrow or human embryo tissue reduces the pain, complexity and ethical considerations associated with these other techniques. As such, the use of ADSCs may offer a safe and effective therapy to treat osteoarthritis and potentially reverse the progression of the disease.
The above image clearly shows cartilage regrowth after using the patients own adipose ( fat ) derived stem cells. A simple liposuction is need to harvest the adipose stem cells. They are activated and injected into the painful joint to help regenerate new tissue and reduce pain for Orthopedic applications.
RESEARCH- British Medical Journal
Photoactivated Platelet-Rich Plasma Therapy for a Traumatic Knee Chondral Lesion
Lifecare Prahran Sports Medicine Centre, Melbourne, Victoria, Australia.
Freitag J, Barnard A, Rotsein A.
To evaluate the effect of combining photoactivation therapy with platelet-rich plasma injections in the treatment of a traumatic chondral lesion of the knee. A 38-year-old man presented with left-knee pain and swelling following a basketball injury. MRI demonstrated a full-thickness lateral tibial plateau chondral flap with subchondral cyst formation and marrow oedema. The patient underwent a course of photoactivated platelet-rich plasma (PAPRP) injections. Patient outcome measures included the numerical pain rating scale and the Western Ontario and McMaster Universities Arthritis Index 3.0 (WOMAC). Following treatment, the patient reported improvement in both pain and function as measured by the numerical pain-rating scale and WOMAC. MRI showed resolution of subchondral bone marrow bruising/oedema. No complications were noted. In this case report, PAPRP injections demonstrated improvement in all recorded outcome measures. Recognising the limitations of a single case report, the results highlight the need for more formal controlled trials to determine the potential use of PAPRP in the treatment of chondral lesions.
Here is the Published Peer Review Clinical Evidence – have a look
PATIENT TESTIMONIAL – see Knee Pain for more patient testimonials on using adipose stem cells to treat painful joints and tendons.
Osteo testimionial from a patient treated using blood stem cells and PRP that lives in Vietnam and treated in Bangkok, Thailand.
I just couldn’t wait to tell you the fantastic news! Today I walked for more than an hour for the first time in several years. It was more than 4km!
And two days ago I also walked for an hour around an old city in Vietnam, on uneven surfaces which has always been more difficult for me.
It’s incredible how unused all my walking muscles are. The only thing that stopped me walking further today was not my knee, but all the muscles I was using to walk which have been dormant for such a long time. I’m also doing yoga every day and this seems to be helping too.
It’s amazing because before I tried these long walks, I was thinking that the stem cells hadn’t helped me at all. Now I realize it’s all the other problems to do with my hip and other muscles which have seized up because I haven’t been walking! And these seem to somehow impact on my ability to walk and pain associated with it. Just five days ago I lamented to Darrell that I didn’t think the stem cells had worked at all because I was hobbling along like an old, old woman.
Now I can’t wait to have the extra stem cell injection when I get back.
Anyway, let’s see how the next injection of my own cells go and how long the benefits last. It seems that nobody is quite sure yet how long the benefit lasts and that older stem cells may have short lived benefits. Do you know anything about this? I gather not because nobody has been doing it long enough to really know.
Anyway, thankyou, thankyou so much for giving me back my mobility! Even for some time, if not permanently
Have a great Christmas and New Year.
Perhaps the next generation of utilizing our own body’s to self heal may utilize a specific light source applied to PRP or platelet rich plasma, bone marrow aspirate (BMAC), blood stem cells or adipose stem cells. After the PRP is prepared and is an a syringe, it is then placed in a device that helps communicate the the cells & steer them towards an anti-inflammatory pathway.
The company AdiStem Ltd., which operates laboratories worldwide, has been researching how monochromatic light of various frequencies and intensities affects populations of mesenchyme stem cells and white blood cells in animals as well as in humans.
Photomodulation and photoactivation using low-level light is used to stimulate and control growth factors (the key component of PRP) in living cells. These lights block the pro-inflammation activity of proteins called cytokines. These Cytokines have been identified as key players in osteoarthritis and chronic tendon injuries and wounds.
Selecting the optimal photomodulaiton wavelength and bandwidth are critical in creating the ideal results. This light stimulation of cells results in the production or inhibition of ATP, which provides the cell with energy and proliferation.
The LED device is used for mesenchyme stem cell activation and the modulation of cytokine release.
Mesenchyme Stem Cells
When mesenchyme stem cells derived from adipose (fat) tissue are removed from a subject most of the cells will lie dormant. Stem cells and progenitor cells in the body are activated by a cascade of physiological processes such as the release of chemokines and growth factor from platelets in the blood. When photons from the LED act on stem cells derived from adipose for 20 minutes we observe an increase in integrin production, interleukin 1 receptor antagonist, thymosin beta 4, vascular endothelial growth factor, and of course, proliferation of adipose stem cells (see Figure 1). Most importantly the clinical results are consistent across age groups, meaning stem cells coming from older patients have good effect on knee pain, shoulder pain and back pain when used on patients.
stem cells derived from adipose after 20-minute LED exposure before plating (right).
Peripheral Blood White Blood Cells
Internal medicine specialists throughout Australia, Thailand and Europe have been utilizing this therapy via photoactivation for many years. White blood cells, exposed to AdiLight-2 for 10-minutes show an anti-inflammatory cytokine response. (See Figure 2). We postulate that using the Adistem light therapy on prepared PRP may potentially result in less post injection pain and quicker recovery by guiding the cells to an anti vs pro-inflammation pathway. We find that some patients have more pain post treatment vs. others & this adjunct allows us to steer patients towards a more speedy recovery. More research is underway to better understand how LED light therapy may further assist our stem cells to heal.
plasma interleukin 1 receptor antagonist peripheral WBCs before (left) and after (right).
THIS PROVES THE LED CAUSES INCREASE IN ANTI-INFLAMMATORY FACTORS
PATIENTS WILL EXPERIENCE LESS PAIN AFTER A STEM CELL OR PRP TREATMENT
CASE REPORT – British Medical Journal – Blood Stem Cells and PRP for Osteoarthritis of the knee. Published Peer Reviewed Clinical Evidence.
To evaluate the effect of combining photo-activation
therapy with platelet-rich plasma injections for the
novel treatment of osteoarthritis
Julien Ben Freitag,1 Adele Barnard2
Objective To evaluate the effect of combining photoactivation
therapy with platelet-rich plasma injections for
the novel treatment of osteoarthritis.
Design We present a case report of osteoarthritis of
the knee treated with photo-activated platelet-rich
plasma injections (PAPRP).
Methods After utilising conventional osteoarthritis
treatment methods a patient underwent a course of
PAPRP injections. The patient outcome was measured
using the numerical pain rating scale (NPRS) and the
Western Ontario and McMaster Universities Arthritis
Results Following treatment the patient reported
improvements in both pain and function as measured by
the NPRS and WOMAC Index, respectively. The patient
was followed up for 18 weeks, at which time no
significant complications were noted.
Conclusions In this case report of osteoarthritis, with
strict control of conventional therapy variables, PAPRP
injections demonstrated improvement in all recorded
outcome measures. The results of this case report
highlight the need to further investigate the use of
PAPRP in the treatment symptomatic knee osteoarthritis.
Pain reduction in patient with Osteoarthritis after blood stem cells and PRP injection three sessions.
Published clinical evidence of improvement of Osteoarthritis using blood stem cells and PRP for knee pain. Pain and stiffness reduced and function and mobility improved.